DNA mutations can demonstrate hereditary risk or predict the likelihood of developing a specific disease. However, they cannot provide phenotypic or quantitative information related to the symptoms of disease or the body’s molecular response. This information is necessary for clinical decision-making and better health outcomes. In contrast, RNA provides a real-time snapshot of what is occurring in the body, allowing for accurate interpretation of DNA variants and a better assessment of the patient’s current health status.
Human cells are constantly being shed from the gastrointestinal lining and passed in the stool. For common gastrointestinal diseases, such as colorectal cancer, inflammatory bowel disease, infectious disease, and irritable bowel syndrome, these cells can uniquely provide early warnings signs and critical information about the condition long before the release of molecular signals into the bloodstream or other parts of the body.
Isolation of human RNA from stool is extremely difficult due to extensive bacterial noise and heavy signal degradation. Bacterial cells far outnumber host cells, making isolation of the eukaryotic signals challenging. Further, the single-stranded nature of RNA makes it less stable and hard to preserve. These characteristics limit the effectiveness of high throughput sequencing and targeted pull-down for downstream applications. Geneoscopy’s proprietary extraction method eliminates bacterial noise, enriches for host signals, and effectively preserves intact RNA.